Abstract:

The study aims to explore the effect of β-glucanase on the activity of defense enzymes in pepper plants and its biological control effect on common pepper soil borne diseases, providing new reference directions for the biological control of pepper soil borne diseases. Pepper seedlings were treated with β-glucanase solution purified by agarose gel chromatography. The activities of SOD, POD, PPO, PAL, CAT, APX and GR in the leaves of pepper seedlings were measured at the seedling stage, the first flowering stage and the fruit setting stage, respectively, and the content of MDA was measured simultaneously. Artificial inoculation was used to investigate the biocontrol effects of different concentrations of β-glucanase treatment on pepper blight, pepper wilt, and pepper anthracnose during the seedling and fruit setting stages. The research results showed that β-glucanase treatment had no effect on GR activity, but promoted the activities of SOD, POD, PPO, PAL, CAT, and APX to varying degrees in peppers, and β-glucanase treatment also had a significant inhibitory effect on MDA content. The control effects of β-glucanase diluted 10-100 times on pepper blight, pepper anthracnose, and pepper wilt were 47.08%-82.23%, 42.35%-70.25%, and 44.21%-65.92%, respectively. β-glucanase can increase the activity of pepper defense enzymes, enhance plant resistance, and has significant biocontrol effects on pepper blight, pepper anthracnose, and pepper wilt. As a biocontrol agent, β-glucanase is environmentally friendly and does not cause insect resistance, making it a promising new biopesticide for controlling pepper soil borne diseases.

Key words: Trichoderma, β-glucanase, pepper, defense enzyme activity, soil-borne disease, biological control