Study on Lignification Dynamics of Dendrocalamus brandisii Shoots Under Low Temperature Preservation After Harvesting

doi:10.11924/j.issn.1000-6850.casb2025-0053

Abstract

Abstract:

In order to provide a theoretical basis for the preservation technology of Dendrocalamus brandisii shoots after picking, the alleviating effects of different low temperature preservation methods on the lignification process of D. brandisii shoots after harvesting were investigated. In this study, the bamboo shoots of D. brandisii from low hilly forest in Guangxi were used as research materials to analyze the changes of lignin, crude fiber content and key enzyme activity of lignin synthesis within 0-10 days after low temperature (4℃) vacuum storage and low temperature sealed storage. The results showed that the contents of lignin and crude fiber in bamboo shoots increased in different degrees within 1-10 d under the two cryopreservation methods. Vacuum treatment could effectively inhibit the synthesis of 43.21% lignin and 28.61% crude fiber. The total phenol content under vacuum treatment always maintained a continuous downward trend, while the total phenolic content decreased under sealing treatment until the 5^th day and showed a certain degree of recovery. The phenylalanine ammonia lyase (PAL) activity of bamboo shoots under the two treatments showed a trend of decreasing first and then slowly increasing within 0-2 d, and the PAL activity under vacuum treatment was significantly lower than that under sealing treatment from the 3^rd day (P<0.001). Principal component analysis and correlation analysis showed that lignin, crude fiber and PAL activity were positively correlated with storage days, and negatively correlated with total phenols. The results showed that total phenol, lignin, crude fiber content and PAL activity were the main response indexes in the lignification process of D. brandisii shoots after harvesting. The physical preservation method of 4℃ low temperature vacuum preservation can effectively delay the lignification process and the rapid decline of taste quality of bamboo shoots.

Key words: Dendrocalamus brandisii shoots, lignification, cryopreservation, enzymatic activity

WEN Yuzhuo, LI Luxi, XU Jingyun, LIANG Xiejun, LUO Wei, BAI Tiandao. Study on Lignification Dynamics of Dendrocalamus brandisii Shoots Under Low Temperature Preservation After Harvesting[J]. Chinese Agricultural Science Bulletin, 2025, 41(24): 135-143.

Figures/Tables 6

References 57

[1]	SHEN Q, KONG F, WANG Q. Effect of modified atmosphere packaging on the browning and lignification of bamboo shoots[J]. Journal of food engineering, 2006, 77(2):348-354.
[2]	夏海涛, 季海宝, 王月英, 等. 勃氏甜龙竹等丛生竹幼竹的高生长规律[J]. 浙江农业科学, 2019, 60(5):807-809. doi: 10.16178/j.issn.0528-9017.20190541
[3]	刘军, 白天道, 陈永力, 等. 南宁低丘松林下3种丛生竹发笋成竹规律[J]. 福建林业科技, 2021, 48(3):19-24.
[4]	赵博, 胡尚连, 刘红. 竹笋采后生理及储藏保鲜技术的研究进展[J]. 竹子学报, 2017, 36(3):66-71.
[5]	RAO X, BARROS J. Modeling lignin biosynthesis: a pathway to renewable chemicals[J]. Trends in plant science, 2023, 29(5):546-559. doi: 10.1016/j.tplants.2023.09.011 pmid: 37802691
[6]	SULIS D B, WANG J P. Regulation of lignin biosynthesis by post-translational protein modifications[J]. Frontiers in plant science, 2020, 11:914. doi: 10.3389/fpls.2020.00914 pmid: 32714349
[7]	陈守江, 姜松. 果蔬中的酚类化合物及其抗氧化作用(综述)[J]. 安徽技术师范学院学报, 2003(2):144-148.
[8]	华晓雨, 陶爽, 孙盛楠, 等. 植物次生代谢产物-酚类化合物的研究进展[J]. 生物技术通报, 2017, 33(12):22-29. doi: 10.13560/j.cnki.biotech.bull.1985.2017-0546
[9]	REN G, WANG B, ZHU X, et al. Cloning, expression, and characterization of miR058 and its target PPO during the development of grapevine berry stone[J]. Gene, 2014, 548(2):166-173.
[10]	YANG C, LIANG Y, QIU D, et al. Lignin metabolism involves Botrytis cinerea BcGs1-induced defense response in tomato[J]. BMC plant biology, 2018, 18(1):103.
[11]	YANG K, ZHU C, ZHANG J, et al. Nitrogen fertilization in bamboo forest accelerates the shoot growth and alters the lignification process in shoots[J]. Industrial crops and products, 2022,187:115368.
[12]	张雄峰, 赵一鹤, 李沁. 茶多酚处理对采后勃氏甜龙竹笋木质化的影响[J]. 西部林业科学, 2022, 51(1):118-124.
[13]	LI C, SUO J, XUAN L, et al. Bamboo shoot-lignification delay by melatonin during low temperature storage[J]. Postharvest biology and technology, 2019,156:110933.
[14]	申德省. 鲜切雷竹笋木质化和褐变的控制研究[D]. 杭州: 浙江工商大学, 2015.
[15]	曾凯芳, 罗晓莉. 赤霉素处理对采后竹笋木质化的影响[J]. 食品科学, 2008(5):457-460.
[16]	CHEN H, LING J, WU F, et al. Effect of hypobaric storage on flesh lignification, active oxygen metabolism and related enzyme activities in bamboo shoots[J]. LWT-food science and technology, 2013, 51(1):190-195.
[17]	LUO Z, FENG S, PANG J, et al. Effect of heat treatment on lignification of postharvest bamboo shoots (Phyllostachys praecox f. prevernalis.)[J]. Food chemistry, 2012, 135(4):2182-2187.
[18]	ZHANG J, MURTAZA A, ZHU L, et al. High pressure CO₂ treatment alleviates lignification and browning of fresh-cut water-bamboo shoots (Zizania latifolia)[J]. Postharvest biology and technology, 2021, 182:111690.
[19]	QI X, JI Z, LIN C, et al. Nitric oxide alleviates lignification and softening of water bamboo (Zizania latifolia) shoots during postharvest storage[J]. Food chemistry, 2020, 332:127416.
[20]	YANG H, ZHOU C, WU F, et al. Effect of nitric oxide on browning and lignification of peeled bamboo shoots[J]. Postharvest biology and technology, 2010, 57:72-76.
[21]	CALEB O, OPARA U, WITTHUHN C. Modified atmosphere packaging of pomegranate fruit and arils: a review[J]. Food and bioprocess technology, 2012, 5:15-30.
[22]	PAREEK S, VALERO D, SERRANO M. Postharvest biology and technology of pomegranate[J]. Journal of the science of food and agriculture, 2015, 95(12):2360-2379. doi: 10.1002/jsfa.7069 pmid: 25565362
[23]	CHETTI M B, DEEPA G T, ANTONY R T, et al. Influence of vacuum packaging and long term storage on quality of whole chilli (Capsicum annuum L.)[J]. Journal of food science and technology, 2014, 51(10):2827-2832.
[24]	陈盈, 金川. 不同处理方法对绿竹笋保鲜效果的影响研究[D]. 杭州: 浙江农林大学, 2015.
[25]	李加兴, 麻成金, 傅伟昌, 等. 篌竹笋真空冷冻干燥工艺研究[J]. 食品研究与开发, 2007(6):80-83.
[26]	张冬梅, 王素雅, 杨茉, 等. Ag-SiO₂-TiO₂纳米包装对新鲜绿竹笋储藏品质的影响[J]. 食品与发酵工业, 2020, 46(15):147-153. doi: 10.13995/j.cnki.11-1802/ts.023910
[27]	王茜, 王曙光, 邓琳, 等. 不同种源版纳甜龙竹竹笋营养成分分析[J]. 西南林业大学学报(自然科学), 2017, 37(5):188-193.
[28]	PEI J L, LI P CH, WANG Q, et al. Comparison of bamboo shoot nutrients of Dendrocalamus brandisii among different provenances[J]. Journal of northwest forestry university, 2018, 33(1):156-161.
[29]	陈松河, 马丽娟, 丁振华, 等. 5种牡竹属笋用竹竹笋营养成分之比较[J]. 竹子学报, 2018, 37(4):4-8,19.
[30]	LI L, WEN Y, XU J, et al. Different parts of a Dendrocalamus brandisii (Munro) Kurz shoot, rather than the shoot’s height, are more indicative of its nutrient properties[J]. Horticulturae, 2024, 10(5):438.
[31]	ITOH T. Lignification of bamboo (Phyllostachys heterocycle Mitf.) during its growth[J]. Holzforschung, 1990, 44(3):191-200.
[32]	蒙兰杨, 黄雪芬, 黄大勇, 等. 6种大型丛生竹造林后的竹笋产量及相关性分析[J]. 中南林业科技大学学报, 2022, 42(2):46-54.
[33]	石伟, 裴艳辉, 张荃. 勃氏甜龙竹竹笋培育研究[J]. 广西林业科学, 2011, 40(4):300-303.
[34]	HATFIELD R, FUKUSHIMA R S. Can lignin be accurately measured?[J]. Crop science, 2005, 45(3):832-839.
[35]	BRAGG C K, SHOFNER F M. A rapid, direct measurement of short fiber content[J]. Textile research journal, 1993, 63(3):171-176.
[36]	PIRIE A, MULLINS M G. Changes in anthocyanin and phenolics content of grapevine leaf and fruit tissues treated with sucrose, nitrate, and abscisic acid[J]. Plant physiology, 1976, 58(4):468-472. doi: 10.1104/pp.58.4.468 pmid: 16659699
[37]	KOUKOL J, CONN E E. The metabolism of aromatic compounds in higher plants[J]. Journal of biological chemistry, 1961, 236(10):2692-2698.
[38]	VENISSE J S, GULLNER G, BRISSET M N. Evidence for the involvement of an oxidative stress in the initiation of infection of pear by Erwinia amylovora[J]. Plant physiology, 2001, 125(4):2164-2172.
[39]	PIZZOCARO F, TORREGGIANI D, GILARDI G. Inhibition of apple polyphenoloxidase (PPO) by ascorbic acid, citric acid and sodium chloride[J]. Journal of food processing and preservation, 1993, 17(1):21-30.
[40]	WICKHAM H, AVERICK M, BRYAN J, et al. Welcome to the tidyverse[J]. Journal of open source software, 2019, 4(43):1686.
[41]	MAIRURA F S, MUSAFIRI C M, MACHARIA J M, et al. Yield-scaled and area-scaled greenhouse gas emissions from common soil fertility management practices under smallholder maize fields in Kenya[J]. Sustainable production and consumption, 2023, 36:292-307.
[42]	WICKHAM H. Ggplot2: Elegant Graphics for Data Analysis[M/OL]. New York: Springer-Verlag, 2016.
[43]	LÊ S, JOSSE J, HUSSON F. FactoMineR: a package for multivariate analysis[J]. Journal of statistical software, 2008, 25(1):1-18.
[44]	KASSAMBARA A, MUNDT F. Factoextra: extract and visualize the results of multivariate data analyses[DB/OL]. https://CRAN.R-project.org/package=factoextra, 2016-04-26.
[45]	DIXON P. Vegan, a package of R functions for community ecology[J]. Journal of vegetation science, 2003, 14(6):927-930.
[46]	YADAV M L, ROYCHOUDHURY B. Handling missing values: a study of popular imputation packages in R[J]. Knowledge-based systems, 2018,160:104-118.
[47]	TAIYUN W, VILIAM S. R package “corrplot”: visualization of a correlation matrix (Version 0.84)[J]. Statistician, 2017, 56:e24.
[48]	LUO Z, XU X, YAN B. Accumulation of lignin and involvement of enzymes in bamboo shoot during storage[J]. European food research and technology, 2008,226:635-640.
[49]	史蔓蔓, 张文, 刘飞翔, 等. 竹笋采后生理生化变化及贮藏保鲜研究进展[J]. 食品科学, 2023, 44(7):331-343.
[50]	张雄峰, 赵一鹤, 李沁. 三种生物保鲜剂处理对采后勃氏甜龙竹笋木质化的影响[J]. 西部林业科学, 2022, 51(5):43-49.
[51]	欧阳光察, 薛应龙. 植物苯丙烷类代谢的生理意义及其调控[J]. 植物生理学通讯, 1988(3):9-16.
[52]	田国忠, 李怀方, 裘维蕃. 植物过氧化物酶研究进展[J]. 武汉植物学研究, 2001(4):332-344.
[53]	BARROS J, SERK H, GRANLUND I, et al. The cell biology of lignification in higher plants[J]. Annals of botany, 2015, 115(7): 1053-1074. doi: 10.1093/aob/mcv046 pmid: 25878140
[54]	WOLTER K, GORDON J. Hormonal control of growth and lignification in relation to peroxidese level and isoengyme expression[J]. Plant physiology, 1972, 49:32.
[55]	王敬文. 采后竹笋老化生理研究[J]. 林业科学研究, 2002(6):687-692.
[56]	李光荣, 刘欢, 张文祥, 等. 生物保鲜剂结合物理技术在果蔬保鲜中应用的研究进展[J]. 食品工业科技, 2021, 42(12):383-388.
[57]	龙国辉, 武鹏雨, 付嘉智, 等. 过氧化物酶调控木质素合成研究进展[J]. 现代农业科技, 2021(23):47-49,54.

指标	Dim-1	Dim-2	Dim-3	Dim-4
CFC	0.9639	0.0812	0.1592	0.0926
LC	0.9055	0.2943	0.0986	-0.0403
TP	-0.5783	0.5626	0.1055	-0.5035
POD	0.0645	-0.0444	0.9500	-0.1788
PAL	0.2252	0.8635	-0.2269	0.0415
PPO	-0.5439	0.3880	0.3527	0.6284
特征值	2.4343	1.3078	1.1245	0.6923
累计贡献率/%	40.6	62.4	81.1	92.6

指标	Dim-1	Dim-2	Dim-3	Dim-4
CFC	0.9639	0.0812	0.1592	0.0926
LC	0.9055	0.2943	0.0986	-0.0403
TP	-0.5783	0.5626	0.1055	-0.5035
POD	0.0645	-0.0444	0.9500	-0.1788
PAL	0.2252	0.8635	-0.2269	0.0415
PPO	-0.5439	0.3880	0.3527	0.6284
特征值	2.4343	1.3078	1.1245	0.6923
累计贡献率/%	40.6	62.4	81.1	92.6