Effect of Vermicompost Substrate Seedling on Tobacco Growth and Metabolic Enzyme Activity

doi:10.11924/j.issn.1000-6850.casb2021-0029

Abstract

Abstract:

Tobacco is used as the experimental material, with the floating seedling culture, the effects of the best ratio of vermicompost (30% vermicompost+ 30% peat+ 20% perlite+ 20% vermiculite) and conventional substrate on the growth and the metabolizing enzyme activities of tobacco in seedling stage, cluster stage and vigorous growth stage were studied, so as to provide technical reference for the application of vermicompost substrate seedling. The results show that, in the seedling stage, the method of vermicompost seedling culture could significantly promote the fresh weight of leaves, total root length, root volume, root diameter, and root activity, compared with the conventional substrate seedling culture. In the cluster stage and vigorous growing stage, the root growth of tobacco with the method of vermicompost seedling culture is better than that with conventional substrate seedling culture, and the root activity could reach significant difference level, increased by 23.50% and 24.98% respectively. However, there is no significant difference in the activity of nitrogen-transformation-related enzymes between the different seedling culture in the cluster stage and the vigorous growing stage. In conclusion, vermicompost substrate could promote the growth of tobacco at seedling stage, cluster stage and vigorous growing stage, and has a more obvious impact on tobacco root growth indicators, so vermicompost substrate is suitable for tobacco seedling in the field.

Key words: vermicompost, tobacco, floating seedling substrate, tobacco growth, enzymatic activity

CLC Number:

S317
S6572

Li Xiaolong, Dong Qingjun, Guo Jianhua, Liu Gaofeng, Tian Yanhua, Zhang Shixiang, Jiao Jiaguo. Effect of Vermicompost Substrate Seedling on Tobacco Growth and Metabolic Enzyme Activity[J]. Chinese Agricultural Science Bulletin, 2021, 37(34): 15-20.

Figures/Tables 6

References 25

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原料	容重/(g/cm³)	孔隙度/%	pH	电导率/(mS/cm)	有机质/(g/kg)	全氮/(g/kg)	全磷/(g/kg)	全钾/(g/kg)
蚓粪	0.75	64.62	6.55	1.72	299.18	22.84	8.72	3.16
泥炭	0.26	60.08	5.39	0.38	308.5	13.34	2.94	1.6
蛭石	0.39	74.42	7.03	0.23	3.61	0.53	0.58	4.46
珍珠岩	0.11	76.11	8.44	0.11	4.73	0.57	7.67	18.27
常规基质	0.24	60.63	6.04	0.17	400.22	5.79	1.56	2.54
蚓粪基质	0.38	62.15	6.82	0.76	394.63	13.9	4.20	5.45

原料	容重/(g/cm³)	孔隙度/%	pH	电导率/(mS/cm)	有机质/(g/kg)	全氮/(g/kg)	全磷/(g/kg)	全钾/(g/kg)
蚓粪	0.75	64.62	6.55	1.72	299.18	22.84	8.72	3.16
泥炭	0.26	60.08	5.39	0.38	308.5	13.34	2.94	1.6
蛭石	0.39	74.42	7.03	0.23	3.61	0.53	0.58	4.46
珍珠岩	0.11	76.11	8.44	0.11	4.73	0.57	7.67	18.27
常规基质	0.24	60.63	6.04	0.17	400.22	5.79	1.56	2.54
蚓粪基质	0.38	62.15	6.82	0.76	394.63	13.9	4.20	5.45

育苗基质	叶鲜重/g	叶干重/g	茎粗/mm	SPAD值
常规基质	5.84±0.01	0.22±0.01	4.83±0.29	29.14±0.70
蚓粪基质	5.31±0.07	0.20±0.01	5.17±0.27	28.44±0.65
T检验	0.01^*	0.15	0.4	0.47

育苗基质	叶鲜重/g	叶干重/g	茎粗/mm	SPAD值
常规基质	5.84±0.01	0.22±0.01	4.83±0.29	29.14±0.70
蚓粪基质	5.31±0.07	0.20±0.01	5.17±0.27	28.44±0.65
T检验	0.01^*	0.15	0.4	0.47

育苗基质	根鲜重/g	根干重/g	总根长/cm	根表面积/cm²	根体积/cm³	根平均直径/mm	根尖数	根系活力/[(µg/(g·h)]
常规基质	0.66±0.02	0.037±0.002	319.14±19.19	45.66±1.86	0.52±0.03	0.46±0.02	377.40±21.36	42.18±1.97
蚓粪基质	0.71±0.01	0.038±0.002	207.09±8.60	33.61±1.35	0.44±0.04	0.52±0.03	317.80±28.71	54.50±2.67
T检验	0.19	0.25	0.001^**	0.008	0.04^*	0.04^*	0.13	0.008^**