Identification, Evolution and Functional Analysis of ApDUF247 Gene Family in Andrographis paniculata

doi:10.11924/j.issn.1000-6850.casb2025-0369

Abstract

Abstract:

The DUF247 gene family plays a significant role in plant growth, development, and stress responses. The purpose of this study aims to reveal the biological functions of the ApDUF247 gene family in Andrographis paniculata and its reaction mechanisms under adverse conditions. The members of the ApDUF247 gene family were identified by bioinformatics method, and their physical and chemical properties, chromosome location, evolutionary relationships, gene structure, cis-regulatory element distribution, expression patterns in different tissues and stress treatments, protein interaction and the expression correlation between ApDUF247 and andrographolide biosynthesis were analyzed. There are twelve ApDUF247 in Andrographis paniculata, which are unevenly distributed on 8 chromosomes, and their structures and functions are diverse. Andrographis paniculata has 11, 7 and 1 pairs of collinear DUF247s genes respectively with Strobilanthes cusia, Sesamum indicum and Arabidopsis thaliana. The promoter region of ApDUF247s is rich in hormone response, photoreaction and transcription factor binding elements. ApDUF247s has tissue-specific expression, which shows different expression patterns under different stress treatments. ApDUF247_8 interacts with 5 serine/threonine protein kinases, ApDUF247_3 interacts with 5 peroxisome-related proteins and 1 E3 ubiquitin lipase, and ApDUF247_6, ApDUF247_9 and ApDUF247_12 are highly correlated with the gene of the ligustrazine biosynthesis pathway (Cor>0.8), and are predicted to be candidate regulatory genes for ligustrazine biosynthesis. This study lays an analytical foundation for preliminarily understanding the biological functions of DUF247 genes in Andrographis paniculata under non-biological stress and their involvement in the regulatory mechanism of andrographolide biosynthesis.

Key words: Andrographis paniculata, DUF247, gene expression, genetics evolution, biological function, abiotic stress, andrographolide, regulatory mechanism, bioinformatics

ZHANG Long, XU Shiqiang, LI Jingyu, LI Xulong, WANG Jihua. Identification, Evolution and Functional Analysis of ApDUF247 Gene Family in Andrographis paniculata[J]. Chinese Agricultural Science Bulletin, 2026, 42(3): 99-108.

Figures/Tables 8

References 27

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基因ID	基因名	氨基酸长度	分子量	等电点	不稳定系数	总平均亲水性	亚细胞定位
CXN00002081-RA	ApDUF247_1	473	52700.14	8.51	48.23	-0.669	细胞质
CXN00004073-RA	ApDUF247_2	529	58971.57	5.84	47.51	-0.198	细胞质
CXN00003503-RA	ApDUF247_3	1680	186638.71	5.34	49.32	-0.115	叶绿体
CXN00012583-RA	ApDUF247_4	859	98005.69	6.31	42.15	-0.352	过氧化物酶体
CXN00000514-RA	ApDUF247_5	545	61351.73	6.01	43.98	-0.128	内质网
CXN00000675-RA	ApDUF247_6	366	41693.45	5.91	47.45	-0.297	细胞质
CXN00024588-RA	ApDUF247_7	518	59123.47	5.96	49.4	-0.309	细胞质
CXN00024587-RA	ApDUF247_8	503	57823.47	6.82	39.33	-0.298	细胞质
CXN00016149-RA	ApDUF247_9	127	14731.38	6.83	34.73	-0.276	细胞质
CXN00021843-RA	ApDUF247_10	160	18016.36	5.49	58.26	-0.642	叶绿体
CXN00014251-RA	ApDUF247_11	219	24515.95	6.38	52.31	-0.288	线粒体
CXN00018330-RA	ApDUF247_12	419	47110	7.6	41.1	-0.024	细胞质

基因ID	基因名	氨基酸长度	分子量	等电点	不稳定系数	总平均亲水性	亚细胞定位
CXN00002081-RA	ApDUF247_1	473	52700.14	8.51	48.23	-0.669	细胞质
CXN00004073-RA	ApDUF247_2	529	58971.57	5.84	47.51	-0.198	细胞质
CXN00003503-RA	ApDUF247_3	1680	186638.71	5.34	49.32	-0.115	叶绿体
CXN00012583-RA	ApDUF247_4	859	98005.69	6.31	42.15	-0.352	过氧化物酶体
CXN00000514-RA	ApDUF247_5	545	61351.73	6.01	43.98	-0.128	内质网
CXN00000675-RA	ApDUF247_6	366	41693.45	5.91	47.45	-0.297	细胞质
CXN00024588-RA	ApDUF247_7	518	59123.47	5.96	49.4	-0.309	细胞质
CXN00024587-RA	ApDUF247_8	503	57823.47	6.82	39.33	-0.298	细胞质
CXN00016149-RA	ApDUF247_9	127	14731.38	6.83	34.73	-0.276	细胞质
CXN00021843-RA	ApDUF247_10	160	18016.36	5.49	58.26	-0.642	叶绿体
CXN00014251-RA	ApDUF247_11	219	24515.95	6.38	52.31	-0.288	线粒体
CXN00018330-RA	ApDUF247_12	419	47110	7.6	41.1	-0.024	细胞质