Response Analysis of OsSIG5 Gene to Phytohormones and Abiotic Stresses in Rice

doi:10.11924/j.issn.1000-6850.casb2024-0420

Abstract

Abstract:

Analysis of the response pattern of sigma factor coding gene OsSIG5 in rice (Oryza sativa) to stress and hormones could provide references for further exploring the functions of OsSIG5 and its homologous genes. Firstly, the bioinformatics of OsSIG5 was performed, and the expression patterns of OsSIG5 in rice under normal and stress conditions were analyzed by quantitative PCR (qRT-PCR). The results showed that the coding region of OsSIG5 was 1503 bp in length, containing 6 exons and encoding 500 amino acids. The OsSIG5 protein had three conserved domains of the σ⁷⁰ family, and phylogenetic analyses showed that the SIG5 homologous protein of monocotyledonous plants was closely related to OsSIG5. The promoter region of OsSIG5 contained 16 plant hormone response cis-elements and 20 environmental stress response cis-elements. OsSIG5 was mainly expressed in rice leaves. The expression of OsSIG5 in leaves was significantly increased under 6-Benzylaminopurine (6-BA) treatment, but significantly decreased under gibberellic acid (GA₃), indole acetic acid (IAA) and abscisic acid (ABA) treatment. In addition, the expression level of OsSIG5 in leaves was significantly decreased after high temperature, low temperature, drought and salt stress treatment. This study showed that OsSIG5 could respond to various hormone signals and abiotic stress signals.

Key words: rice, sigma factors, OsSIG5, phylogenetic analysis, cis-acting element analysis, expression pattern, plant hormones, abiotic stress

ZHAN Jiatao, MIAO Leyi, JI Zixian, WANG Yiting, LU Yue, TAN En, MA Shuaipeng, WANG Limin. Response Analysis of OsSIG5 Gene to Phytohormones and Abiotic Stresses in Rice[J]. Chinese Agricultural Science Bulletin, 2025, 41(14): 32-40.

Figures/Tables 8

References 37

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引物名称	引物序列(5’-3’)
OsSIG5-qF	TCGACTGGAAGCGGATGAAG
OsSIG5-qR	CGCCTCATCTGAAGAACGGA
Actin1-qF	GCATCTCTCAGCACATTCCA
Actin1-qR	ACCACAGGTAGCAATAGGTA

引物名称	引物序列(5’-3’)
OsSIG5-qF	TCGACTGGAAGCGGATGAAG
OsSIG5-qR	CGCCTCATCTGAAGAACGGA
Actin1-qF	GCATCTCTCAGCACATTCCA
Actin1-qR	ACCACAGGTAGCAATAGGTA

顺式作用元件	数量
光响应	18
生长素响应	3
低温响应	1
水杨酸响应	1
脱落酸响应	10
厌氧诱导相关	1
茉莉酸甲酯反应响应	2
根特异性相关	1
MYB结合位点相关	2

顺式作用元件	数量
光响应	18
生长素响应	3
低温响应	1
水杨酸响应	1
脱落酸响应	10
厌氧诱导相关	1
茉莉酸甲酯反应响应	2
根特异性相关	1
MYB结合位点相关	2