Effects of Low Temperature and Natural Light on Release of Diapause in 4th Instar Nymphs of Sphedanolestes impressicollis

doi:10.11924/j.issn.1000-6850.casb2025-0715

Abstract

Abstract:

Artificially reared 4th instar nymphs of Sphedanolestes impressicollis are prone to diapause, which seriously restricted its reproductive efficiency. To enhance breeding efficiency, it is necessary to artificially break diapause. In the insect laboratory of the Hebei Academy of Forestry Sciences, 4th instar nymphs of Sphedanolestes impressicollis were subjected to 5℃ (no light, 50% humidity) low-temperature treatment. Two treatments of constant temperature (5℃) and fluctuating temperature (15℃→5℃→15℃) were conducted, as well as natural light exposure after low-temperature treatment. The developmental progress of the 4th instar nymphs of Sphedanolestes impressicollis was regularly monitored. The results showed that 5℃ low temperature and natural light exposure could effectively break diapause in 4th instar nymphs of Sphedanolestes impressicollis, with normal development observed. Fluctuating temperature treatment significantly outperformed constant temperature treatment, with an average generation time of 90.8 days, improving the efficiency of artificial breeding for the 4th instar nymphs of Sphedanolestes impressicollis. The research of induction and termination of diapause in 4th instar nymphs of Sphedanolestes impressicollis contribute to understanding the seasonal adaptation patterns and mechanisms of this species. Practically, targeted induction or termination of diapause can promote large-scale propagation and storage, providing technical support for field applications.

Key words: Sphedanolestes impressicollis, low temperature, natural light, diapause, relieve

CLC Number:

S476+.2

LI Mengchai, LIU Manguang, XU Lian, TIAN Long, ZHANG Dongfeng, ZHAO Junning. Effects of Low Temperature and Natural Light on Release of Diapause in 4th Instar Nymphs of Sphedanolestes impressicollis[J]. Chinese Agricultural Science Bulletin, 2026, 42(16): 195-199.

Figures/Tables 4

References 32

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发育历期/d	25~15℃	5℃	5~15℃	解除滞育（4龄）	5龄
1~2号	0 d	14 d	0 d	38.1aA	29.3aA
3~8号	7 d	14 d	7 d	31.7aA	16.8bB
CK（气候箱内）	25~20℃人工气候箱			76bB	89.5cC

发育历期/d	25~15℃	5℃	5~15℃	解除滞育（4龄）	5龄
1~2号	0 d	14 d	0 d	38.1aA	29.3aA
3~8号	7 d	14 d	7 d	31.7aA	16.8bB
CK（气候箱内）	25~20℃人工气候箱			76bB	89.5cC

发育历期	4龄（3月6日）	5龄（3月20日）	成虫（4月2日）
光照处理/d	14.6	13.7	出现
CK（气候箱内）		未见5龄

发育历期	4龄（3月6日）	5龄（3月20日）	成虫（4月2日）
光照处理/d	14.6	13.7	出现
CK（气候箱内）		未见5龄