Control Effect of Bio-organic Fertilizer Containing Pseudomonas fluorescens on Bacterial Wilt Disease in Facility Eggplants and Its Role in Yield Enhancement and Quality Improvement

doi:10.11924/j.issn.1000-6850.casb2025-1039

Abstract

Abstract:

The study aimed to explore the control effect of bio-organic fertilizer containing Pseudomonas fluorescens on bacterial wilt of facility eggplant, and its influences on yield, quality and soil properties, so as to provide theoretical basis and technical support for scientific fertilization in continuous cropping of facility eggplant. Field experiment was conducted with three treatments, including conventional fertilization (CK), basal application of inactivated bio-organic fertilizer (K₁), and basal application of bio-organic fertilizer containing Pseudomonas fluorescens (K₂). The application effects of the bio-organic fertilizer were analyzed by determining eggplant growth indicators, fruit quality, incidence of bacterial wilt, and relevant soil properties. The results indicated that the K₂ treatment significantly enhanced eggplant yield and quality, and exerted positive effects on soil enzyme activities and rhizosphere microbial biomass. Compared with CK, K₂ treatment significantly increased fruit single weight, yield, soluble protein content, soluble sugar content, and vitamin C content by 10.58%, 7.79%, 31.47%, 25.85%, and 17.48% respectively, while reducing cellulose content by 65.52%. The activities of soil urease, catalase, sucrase, and acid phosphatase increased by 50.00%, 42.84%, 50.23%, and 30.04% respectively, and the populations of rhizosphere actinomycetes, fungi, and bacteria increased by 55.71%, 78.00%, and 15.57% respectively. The bio-organic fertilizer containing Pseudomonas fluorescens can achieve disease control, yield improvement and quality optimization of facility eggplant under continuous cropping by optimizing soil microecological environment and enhancing plant photosynthetic capacity, providing an effective pathway for resolving the obstacles associated with the continuous cropping of facility eggplant.

Key words: Pseudomonas fluorescens, bio-organic fertilizer, facility eggplant, application effect

CLC Number:

S144.9其他

HUANG Jian. Control Effect of Bio-organic Fertilizer Containing Pseudomonas fluorescens on Bacterial Wilt Disease in Facility Eggplants and Its Role in Yield Enhancement and Quality Improvement[J]. Chinese Agricultural Science Bulletin, 2026, 42(14): 166-171.

Figures/Tables 4

References 32

[1]	王菲, 李银科, 王正银, 等. 缓释复合肥对茄子产量和不同采果期品质的影响[J]. 土壤学报, 2015, 52(2):355-363.
[2]	卢家柱, 赵贵宾, 颉建明, 等. 不同施氮量对茄子产量、品质及肥料利用率的影响[J]. 华北农学报, 2016, 31(3):205-211. doi: 10.7668/hbnxb.2016.03.030
[3]	马兆红, 连勇, 方木壬, 等. 从生产市场需求谈我国茄子品种的变化趋势[J]. 中国蔬菜, 2017(9):1-6.
[4]	孙义, 居正英, 林玲, 等. 茄子黄萎病生防内生细菌29-12发酵条件的初步研究[J]. 江苏农业学报, 2008, 24(4):425-430.
[5]	陈廷钦. 土壤调理剂及应用进展[J]. 云南大学学报(自然科学版), 2011, 33(增刊1):338-342.
[6]	杨尚东, 李荣坦, 谭宏伟, 等. 长期单施化肥和有机无机配合条件下红壤蔗区土壤生物学性状及细菌多样性差异[J]. 植物营养与肥料学报, 2016, 22(4):1024-1030.
[7]	WANG Z, LUO W, CHENG S, et al. Ralstonia solanacearum-a soil borne hidden enemy of plants:research development in management strategies, their action mechanism and challenges[J]. Frontiers in plant science, 2023, 14:1141902. doi: 10.3389/fpls.2023.1141902 URL
[8]	JAMES C R. Making greater use of introduced microorganisms for biological control of plant pathogens[J]. Annual review of phytopathology, 1993, 31:53-80. pmid: 18643761
[9]	袁英英, 李敏清, 胡伟, 等. 生物有机肥对番茄青枯病的防效及对土壤微生物的影响[J]. 农业环境科学学报, 2011, 30(7):1344-1350.
[10]	曹明慧, 冉炜, 杨兴明, 等. 烟草黑胫病拮抗菌的筛选及其生物效应[J]. 土壤学报, 2011, 48(1):151-159.
[11]	SHAH A, NAZARI M, ANTAR M, et al. PGPR in agriculture: A sustainable approach to increasing climate change resilience[J]. Frontiers in sustainable food systems, 2021, 5:211-232.
[12]	PRABHUKARTHIKEYAN S R, KEERTHANA U, RAGUCHANDER T. Antibiotic-producing Pseudomonas fluorescens mediates rhizome rot disease resistance and promotes plant growth in turmeric plants[J]. Microbiological research, 2018, 210:65-73. doi: S0944-5013(17)31278-8 pmid: 29625661
[13]	SAH S, KRISHNANI S, SINGH R. Pseudomonas mediated nutritional and growth promotional activities for sustainable food security[J]. Current research in microbial sciences, 2021, 2:100084. doi: 10.1016/j.crmicr.2021.100084 URL
[14]	赵娜, 蔡昆争, 汪国平, 等. 家畜堆肥诱导番茄对青枯病的抗性及其生理机制[J]. 农业环境科学学报, 2008, 27(5):2058-2063.
[15]	鲍士旦. 土壤农化分析(第三版)[M]. 北京: 中国农业出版社, 1981:14-20.
[16]	VESTBERG M, KUKKONEN S, SAARI K, et al. Microbial inoculation for improving the growth and health of micropropagated strawberry[J]. Applied soil ecology, 2004, 27(3):243-258. doi: 10.1016/j.apsoil.2004.05.006 URL
[17]	曹建康, 姜微波, 赵玉梅. 果蔬采后生理生化实验指导[M]. 北京: 中国轻工业出版社, 2007:28-29,57-59,68-70.
[18]	高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006:200-201.
[19]	关松荫. 土壤酶及其研究法[M]. 北京: 中国农业出版社, 1986:274-324.
[20]	沈萍, 陈向东. 微生物学实验(5版)[M]. 北京: 高等教育出版, 2018:56,89.
[21]	SINGH R P, TIWARI S, SINGH M, et al. Important diseases of greenhouse crops and their integrated management: A review[J]. Journal of entomology and zoology studies, 2020, 8(1):962-970.
[22]	黄剑, 王健, 李晓阳, 等. 氨基丁酸与有机肥料配施对连作马铃薯产量、品质和土壤活性的影响[J]. 天津农业科学, 2025, 31(7):79-84.
[23]	刘晓. 微生物菌肥在农业生产中的应用研究[J]. 河南农业, 2021,(17):14-15.
[24]	TANG T T, SUN X, LIU Q, et al. Treatment with organic manure inoculated with a biocontrol agent induces soil bacterial communities to inhibit tomato fusarium wilt disease[J]. Frontiers in microbiology, 2023, 13:1006878. doi: 10.3389/fmicb.2022.1006878 URL
[25]	李桂花, 谭琳, 沈程文, 等. 茶树根腐病拮抗放线菌分离、鉴定及生防潜能研究[J]. 植物保护, 2024, 50(5):87-96,204.
[26]	WANG B, YANG B, PENG H, et al. Genome sequence and comparative analysis of fungal antagonistic strain Bacillus velezensis LJBV19[J]. Folia microbiologica, 2023, 68(1):73-86. doi: 10.1007/s12223-022-00996-z
[27]	WU H S, YANG X N, FAN J Q, et al. Suppression of Fusarium wilt of watermelon by a bio-organic fertilizer containing combinations of antagonistic microorganisms[J]. BioControl, 2009, 54:287-300. doi: 10.1007/s10526-008-9168-7 URL
[28]	蔡燕飞, 廖宗文, 章家恩, 等. 生态有机肥对番茄青枯病及土壤微生物多样性的影响[J]. 应用生态学报, 2003, 14(3):349-353.
[29]	杨囡君, 刘新社, 决超. 不同土壤改良措施对连作黄瓜幼苗生理特性和产量及品质的影响[J]. 江苏农业科学, 2024, 52(8):144-150.
[30]	薛磊, 凡莉莉, 李云鸽, 等. 生物菌肥不同施用量对鼓节竹发笋期生长和光合特性的影响[J]. 热带作物学报, 2018, 39(7):1332-1337.
[31]	郑国保, 孔德杰, 张源沛, 等. 不同灌溉定额对设施茄子光合特性和产量的影响[J]. 节水灌溉, 2010(12):28-30,34.
[32]	任金凤. 接种荧光假单胞菌对花生根腐病的防治效应研究[D]. 徐州: 江苏师范大学, 2024.

处理	单果重/g	产量/(kg/hm²)	SPAD值	病情指数/%	防治效果/%
CK	176.60±5.16b	76517.32±393.74c	33.30±0.47c	5.37±0.18a	—
K₁	181.49±3.14ab	78867.45±547.30b	41.22±0.50b	4.93±0.09b	8.19
K₂	195.29±5.31a	82480.56±223.97a	48.70±0.31a	3.57±0.09c	33.52

处理	单果重/g	产量/(kg/hm²)	SPAD值	病情指数/%	防治效果/%
CK	176.60±5.16b	76517.32±393.74c	33.30±0.47c	5.37±0.18a	—
K₁	181.49±3.14ab	78867.45±547.30b	41.22±0.50b	4.93±0.09b	8.19
K₂	195.29±5.31a	82480.56±223.97a	48.70±0.31a	3.57±0.09c	33.52

处理	可溶性蛋白含量/(mg/g)	可溶性糖含量/%	纤维素含量/(g/g）	维生素C含量/(mg/100 g）
CK	2.51±0.04b	2.36±0.05b	0.029±0.001a	11.21±0.04c
K₁	2.71±0.05b	2.88±0.02a	0.013±0.001b	12.45±0.04b
K₂	3.30±0.08a	2.97±0.03a	0.010±0.002b	13.17±0.09a

处理	可溶性蛋白含量/(mg/g)	可溶性糖含量/%	纤维素含量/(g/g）	维生素C含量/(mg/100 g）
CK	2.51±0.04b	2.36±0.05b	0.029±0.001a	11.21±0.04c
K₁	2.71±0.05b	2.88±0.02a	0.013±0.001b	12.45±0.04b
K₂	3.30±0.08a	2.97±0.03a	0.010±0.002b	13.17±0.09a

处理	脲酶活性/ [mg/(g·d)]	过氧化氢酶活性/ [mL/(g·d)]	蔗糖酶活性/ [mg/(g·d)]	酸性磷酸酶活性/ [mg/(g·d)]	放线菌总菌落数/ (×10⁵ CFU/g)	真菌总菌落数/ (×10³ CFU/g)	细菌总菌落数/ (×10⁶ CFU/g)
CK	1.12±0.05b	8.94±0.07c	10.95±0.16c	11.22±0.07c	22.17±0.09c	23.68±0.08c	51.38±0.1c
K₁	1.25±0.03b	9.39±0.14b	11.71±0.03b	12.15±0.03b	27.17±0.05b	31.03±0.06b	52.84±0.07b
K₂	1.68±0.07a	12.77±0.09a	16.45±0.04a	14.59±0.07a	34.52±0.08a	42.15±0.12a	59.38±0.34a