Abstract: Using super sweet corn inbreeding lines S1, S2, S3 and hybrid Yue Tian No.3 as materials, the efficient regeneration systems were established. The research showed that the genotype and length of immature embryo of super sweet corn were the key factors influencing the embryogenic callus development, 0.5-1.2mm immature embryo were better for embryogenic callus inducing. contents of sugar and cytokinins in medium were the main factors for plantlet regeneration, low content of sugar( 20g/L) and 6-BA（2mg/L）or Zeatin (3 mg/L) are more fit to the regeneration, and the rate of regeneration could be increased 1.68 times when callus treated with ABA in sub-culture for 3 weeks. IBA could improve plantlet rooting, and the content of 3.0mg/L was the best.