Abstract: 10 microsatillite sites were used for DNA amplification To search for markers those have significant relation with body weight trait in Nanjiang Yellow goat population, which have great phenotypic variation on body weight. Then these sites primeers were applied to amplify genomic DNA in random population, and calculated effect of marker and substitution according to general linear model. The result indicated that, 186bp and190bp of BM3413, 109bp of MFA70, 178bp and 184bp of INRA063, 110bp of MB066, 176bp of MB3501, these markers showed significant positive correlation with body weight trait, the correlate coefficient was in the region of 0.5180~0.7375. These markers, 182bp of BM3413, 115bp of MFA70, 98bp of MB066, showed significant negative correlation with body weight trait, correlation coefficient was in the region of t-0.6539~-0.388.