Study on Pest Susceptibility to Multi-bioinsecticidal-toxin BtA

Abstract

Abstract: Multi-bioinsecticidal-toxin BtA is a bioconjugation product with multiple acting-sites, formed by biochemical bond-linking reaction between two biotoxins, protoxin of Bacillus thuringiensis and Abamectin, through the amino and carboxyl conjugator EDC. The variation of pest susceptibility to Multi-bioinsecticidal-toxin BtA was evaluated by studying the decline and recovery of susceptibility of Plutella xylostella L. to BtA and the susceptibility diversity of P. xylostella populations collected from different regions. The results showed that the susceptibilities of the relatively susceptible lab population were stable to BtA and its two reactants, Bt crystal and Abamectin, with LC50s of 0.0004-0.0020mg/ml. Under the selection pressure, the pest susceptibility reduced, with Resistance Ratio (RR) of 2.29 to BtA after 2 generations comparing with 8.35 and 22.5 to Bt crystal and Abamectin respectively. While the field population was released from the selection pressure, the pest susceptibility increased 4.7-fold to BtA after 2 generations in comparison to 1.28-fold Bt crystal and 2.00-fold to Abamectin. The P. xylostella from seven regions of Fujian province represented polymorphism of susceptibility, while the RRs to BtA, Bt crystal and Abamectin were 1.18~6.85, 1.22~23.93 and 1.58~4.77 respectively. The result of cluster analysis displayed that P. xylostella in middle part of Fujian province had lower susceptibility. It was concluded that the Multi-bioinsecticidal-toxin BtA could help to delay the resistance development, reduce the resistance and rapidly recovery of pest susceptibility.

Zhu Yujing,, Liu Bo, Cetin Sengonca, Ruan Chuanqing, Lin Kanmei, Mao Naihe,. Study on Pest Susceptibility to Multi-bioinsecticidal-toxin BtA[J]. Chinese Agricultural Science Bulletin, 2005, 21(12): 316-316.

References

1 张国洲.害虫抗药性及其治理[J].安徽农业科学,2002,30(4):512~514
2 Shelton A M, Kroening M K, Eigenbrode S D, et al. Diamondback moth (Lepidoptera: Plutellidae) contamination of cabbage transplants and the potential for insecticide resistance problems[J]. J Entomol Sci, 1996, 31: 347~354
3 Iqbal, M., Wright, D. J. Evaluation of resistance, cross-resistance and synergism of abamectin and teflubenzuron in a multi-resistant field population of Plutella xylostella (Lepidoptera: Plutellidae) [J]. B Entomol Res, 1997, 87: 481~486
4 Zhao, J, Collins H L, Tang J D, et al. Development and characterization of diamondback moth resistance to transgenic broccoli expressing high levels of Cry1C[J]. Appl Environ Microb, 2000, 66: 3784~3789
5 刘波,朱育菁,Sengonca C,等.害虫抗药性治理策略——多位点杀虫毒素的研究[J].农药,2004,43(11): 492~496
6 Liu B, Sengonca C. Conjugation of δ-endotoxin from Bacillus thuringiensis with abamectin of Streptomyces avemitilis as a new type of biocide GCSC-BtA in control of agricultural insect pests[J]. J Pest Sci. 2003, 76: 44~49
7 Liu B, Sengonca C. Biotechnological development of GCSC-BtA as a new type of biocide[M]. Germany: Cuvillier Verlag G?ettingen, 2004.1~3
8 刘波,苑宝玲,Sengongca C,等.高效生物杀虫剂藕合技术的研究[J].中国生物防治,2000,12(增刊): 28~34
9 Finney D J. Probit Analysis[M]. 3rd edn. Cambridge Univ Press, Cambridge, UK, 1971.333
10 唐启义,冯明光.实用统计分析及其计算机处理平台[M].北京:中国农业出版社,1997.46~70
11 黄剑,吴文君.小菜蛾抗药性研究进展[J].贵州大学学报(自然科学版),2003,20(1): 97~104
12 Liu, Y, Tabashnik B E, Liu Y B. Inheritance of resistance to the Bacillus thuringiensis toxin Cry1C in the diamondback moth[J]. Appl Environ Microb, 1997, 63:2218~2223
13 何婕和,张雪燕.小菜蛾对阿维菌素的抗性形成规律[J].西南农业学报,2000,13(2): 67~70
14 李凤良,李忠英,韩招久,等.小菜蛾对溴氰菊酯的抗性消退与抗性恢复动态[J].植物保护学报,2004,31(1): 79~84
15 吴刚,尤民生,赵士熙.小菜蛾抗性稳定性及抗性治理对策研究[J].农药学学报,2001,3(1): 83~86