1 Lassner M, Bedbrook J. Directed molecular evolution in plant improvement. Curr Opin Plant Biol., 2001, 4(2):152~156
2 Lassner M W, McElroy D. Directed molecular evolution: bridging the gap between genomics leads and commercial products. OMICS., 2002,6(2):153~162
3 Owen M D, Zelaya I A. Herbicide-resistant crops and weed resistance to herbicides. Pest Manag Sci., 2005, 61(3): 301~311
4 Stalker D M, McBride K F, Malyj M D. Herbicide resistance in transgenic plants expressing a bacterial detoxification gene. Science., 1988, 242: 419~423
5 Thompson C J, Movva N R, Tizard R, et al. Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. EMBO J., 1987, 6: 2519~2523
6 Pline W A, Wilcut J W, Duke S O, et al. Tolerance and accumulation of shikimic acid in response to glyphosate applications in glyphosate-resistant and nonglyphosate-resistant cotton (Gossypium hirsutum L.). J Agric Food Chem., 2002, 50(3): 506~512
7 Castle L A, Siehl D L, Gorton R, et al. Discovery and directed evolution of a glyphosate tolerance gene. Science., 2004, 304(5674): 1151~1154
8 He M, Nie Y F, Xu P. A T42M substitution in bacterial 5-enolpyruvylshikimate-3- phosphate synthase (EPSPS) generates enzymes with increased resistance to glyphosate. Biosci Biotechnol Biochem., 2003, 67(6): 1405~1409
9 Siehl D L, Castle L A, Gorton R, et al. Evolution of a microbial acetyltransferase for modification of glyphosate:a novel tolerance strategy. Pest Manag Sci., 2005, 61(3): 235~240
10 Dixon D P, McEwen A G, Lapthorn A J, et al. Forced evolution of a herbicide detoxifying glutathione transferase. J Biol Chem., 2003, 278(26): 23930~23935
11 Van Rie J. Bacillus thuringiensis and its use in transgenic insect control technologies. Int J Med Microbiol., 2000, 290(4~5):463~169
12 Peng R, Xiong A, Li X, et al. A delta-endotoxin encoded in Pseudomonas fluorescens displays a high degree of insecticidal activity. Appl Microbiol Biotechnol., 2003, 63(3): 300~306
13 Estruch J J, Carozzi N B, Desai N, et al. Transgenic plants: an emerging approach to pest control. Nat Biotechnol., 1997, 15(2): 137~141
14 Haq S K, Atif S M, Khan R H. Protein proteinase inhibitor genes in combat against insects, pests, and pathogens: natural and engineered phytoprotection. Arch Biochem Biophys., 2004, 431(1): 145~159
15 Guerrero A, Rosell G. Enzyme inhibitors in biorational approaches for pest control. Mini Rev Med Chem., 2004, 4(7): 757~767
16 Gerhardson B. Biological substitutes for pesticides. Trends Biotechnol., 2002, 20(8): 338~343
17 McPherson M J, Harrison D J. Protease inhibitors and directed evolution: enhancing plant resistance to nematodes. Biochem Soc Symp., 2001, 68: 125~142
18 Campbell M A, Fitzgerald H A, Ronald P C. Engineering pathogen resistance in crop plants. Transgenic Res., 2002, 11(6): 599~613
19 Stuiver M H, Custers J H. Engineering disease resistance in plants. Nature., 2001, 411(6839): 865~868
20 Bernal A J, Pan Q, Pollack J, et al. Functional analysis of the plant disease resistance gene Pto using DNA shuffling. J Biol Chem., 2005, 280(24): 23073~23083
21 王国栋,陈晓亚.漆酶的性质、功能、催化机理和应用.植物学通报,2003,20(4):469~475
22 Bulter T, Alcalde M, Sieber V, et al. Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution. Appl Environ Microbiol., 2003, 69(2): 987~995
23 黄大昉.农业微生物基因工程研究与展望.农业生物技术学报,2003,11(2):111~114
24 Lehmann M, Loch C, Middendorf A, et al. The consensus concept for thermostability engineering of proteins: further proof of concept. Protein Eng., 2002, 15(5): 403~411
25 Mullaney E J, Daly C B, Kim T, et al. Site-directed mutagenesis of Aspergillus niger NRRL 3135 phytase at residue 300 to enhance catalysis at pH 4.0. Biochem Biophys Res Commun., 2002, 297(4): 1016~1020
26 Tomschy A, Brugger R, Lehmann M, et al. Engineering of phytase for improved activity at low pH. Appl Environ Microbiol., 2002, 68(4): 1907~1913
27 Mullaney E J, Daly C B, Ullah A H. Advances in phytase research. Adv Appl Microbiol., 2000, 47: 157~199
28 彭日荷,熊爱生,李贤,等.应用毕氏酵母高效表达耐高温植酸酶.生物化学与生物物理学报,2002,34(6):725~730
29 Xiong A S, Yao Q H, Peng R H, et al. Isolation, characterization, and molecular cloning of the cDNA encoding a novel phytase from Aspergillus niger 113 and high expression in Pichia pastoris. J Biochem Mol Biol., 2004, 37(3): 282~291
30 Xiong A S, Yao Q H, Peng R H, et al. High level expression of a recombinant acid phytase gene in Pichia pastoris. J Appl Microbiol., 2005, 98(2): 418~428
31 Peng R H, Yao Q H, Xiong A S, et al. Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola. Plant Cell Reports., 2005, in press
32 Berk H, Lebbink R J. High-throughput screening of mutant alpha-amylase libraries for increased activity at 129 degrees C. Methods Mol Biol., 2003, 230: 127~135
33 Verhaert R M, Beekwilder J, Olsthoorn R, et al. Phage display selects for amylases with improved low pH starch-binding. J Biotechnol., 2002, 96(1): 103~118
34 Wang T, Liu X, Yu Q, et al. Directed evolution for engineering pH profile of endoglucanase III from Trichoderma reesei. Biomol Eng., 2005, 22(1): 89~94
35 Murashima K, Doi R H. Selection of heat-stable Clostridium cellulovorans cellulases after in vitro recombination. Methods Mol Biol., 2003, 230: 231~237
36 Murashima K, Kosugi A, Doi R H. Thermostabilization of cellulosomal endoglucanase EngB from Clostridium cellulovorans by in vitro DNA recombination with non-cellulosomal endoglucanase EngD. Mol Microbiol., 2002, 45(3): 617~626
37 Kim Y S, Jung H C, Pan J G. Bacterial cell surface display of an enzyme library for selective screening of improved cellulase variants. Appl Environ Microbiol., 2000, 66(2): 788~793
38 McCarthy J K, Uzelac A, Davis D F, et al. Improved catalytic efficiency and active site modification of 1,4-beta-D-glucan glucohydrolase A from Thermotoga neapolitana by directed evolution. J Biol Chem., 2004, 279(12): 11495~11502
39 Palackal N, Brennan Y, Callen W N, et al. An evolutionary route to xylanase process fitness. Protein Sci., 2004, 13(2): 494~503
40 nami M, Morokuma C, Sugio A, et al. Directed evolution of xylanase J from alkaliphilic Bacillus sp. strain 41M-1: restore of alkaliphily of a mutant with an acidic pH optimum. Nucleic Acids Res Suppl., 2003, 3: 315~316
41 Chen Y L, Tang T Y, Cheng K J. Directed evolution to produce an alkalophilic variant from a Neocallimastix patriciarum xylanase. Can J Microbiol., 2001, 47(12): 1088~1094
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