Abstract: An experiment was conducted with mixed materials of chicken excrement and wheat straw to study the changes of some hydrolyses activities, pH value, and election conductance rate (EC) during an aeration composting process in a static state. Results showed that the activities of cellulose enzyme and sucrose enzyme under the condition of adding microorganism fungus had the same tendency in variation with that of no microorganism fungus, the active peak values of the two kinds of enzyme were higher than the treatment of no microorganism fungus, and their peak values were up to 0.457mg glucose/g·24h and 87.836mg glucose/g·24h respectively, the appearances of the two kinds of enzyme peak values were earlier than the treatment of no microorganism fungus, and among them cellulose enzyme was 2d earlier and sucrose enzyme was 6d earlier. Applying microorganism fungus can improve urea enzyme activity and its peak value, reduce the value of pH, and increase the value of EC at the same time.