Abstract: Acanthopanax senticosus(Rupr Emaxim) Harms is a kind of defoliated shrub which belongs to Araliaceae Eleutherococcus. In recent years many kinds of julibrissins are isolated from the roots and leaves of Acanthopanax senticosus. Chemical pharmacology research shows that Acanthopanax senticosus is a kind of extremely precious medicine resources, and its functions include antitumor, resist fatigue, anti-ageing, at the same time, it can protect cardiovascular effectively, and improve immunity of human body. With the development of modern medicine studying rapidly, the demands for the plant for medinice increase day by day, so that the domestic and international experts pay more and more attention to Acanthopanax senticosus and its applications. Offering of medicines clinically depends on drawing from the roots and stems of Acanthopanax senticosus mainly; this makes it close to extinction. The imbalance between supply and demand of market is conspicuous, and products of the plant can't meet demands of research of the antineoplastic. Technology on tissue culture and rapid propagation of Acanthopanax senticosus can breed out a large number of nursery stocks as fine individuals. As xylophyta, it is very difficult for rooting induction, so the key about tissue culture is how to improve rooting rate, and this need remain to study further. It is demonstrated by the experiment that callus shoot and rooting induction of Acanthopanax senticosus are influenced by different hormone combinations of culture media. BZ+NAA3.0+6-Ba2.0 is the favorable medium for differentiation of Acanthopanax senticosus tissue culture, and BZ+IBA0.2 is the one for rooting. Differentiation ability of Acanthopanax senticosus callus is influenced by the type of explants and holding time of suspension culture mainly. Differentiation ability of Acanthopanax senticosus callus that is obtained from leaves is the best. After 30 days for suspension culture, differentiation rate of callus get to 73.62%, and differentiation coefficient of regeneration bud is 7.5. Differentiation ability of Acanthopanax senticosus callus reduces with extension of suspension holding time of it.