Abstract: Different genotype of maize inbred lines (7922, 8902, 4112, 340, 853) were used to study the relationship between somatic embryo formation and regeneration frequency. Results showed that 7922 had a regeneration frequency of 74.4% in somatic embryo pathway, while 8902, 4112, 340 and 853 had a regeneration frequency of 8.2%, 12.7%, 0%, 11.88% in adventitious buds pathway. It concluded that 7922 was the best genetic transformation receptor in this experiment. Mutation frequency would be accumulated with increasing somatic callus subculture times, therefore, subculture times should be decreased for high transformation frequency. A aggregated construct with bar, cryIA(a) and pta, named p3300-bt-pta, was transformed into 7922 calli by PDS-100. Herbicide (phosphinothricin, PPT3mg/L) resistance screening indicated that there were 22 individual putative transformed plants with herbicide resistance in total. PCR detection further revealed that there were 7 individuals with three resistant genes integrated and cointegration frequency was 31.8%. In conclusion, it is feasible to introduce multi-resistant-genes into maize to get transgenic maize with multi-resistance to Lepidoptera insect and Homoptera insect in a single transgenic event.