Abstract: Persimmon fruits harvested from different coloring period were used to extract carotenoid and then the extracted carotenoid was treated with saponification. The pigment sample was analyzed through HPLC in terms of characterization and quantification. The results showed that pigment in persimmon fruit of collection period was mainly made up of zeaxanthin, β-cryptoxanthin, β-carotene, Lutein and Lycopine. There was almost no difference in terms of carotenoid composition between peel and flesh, but the ratio was different. The total content of carotenoid in peel was about six--eleven times more than that in flesh. The maximum composition was zeaxanthin, β-Cryptoxanthin was the next, and the third one wasβ-carotene in peel. While the maximum composition wasβ-cryptoxanthin, Zeaxanthin was the next, and the third one wasβ-carotene in flesh. It showed that primary component in flesh isβ-Cryptoxanthin and the accumulation of β-Cryptoxanthin in persimmon fruit was influenced by temperature.