Analysis of DNA Polymorphism in Peanut Cultivars Using New Microsatellite Markers

Abstract

Abstract: SSR primers published for peanut, along with those from a strategy involving hybridization enrichment, library construction were evaluated using 12 peanut cultivars mainly from Shandong. Cluster analysis was carried out based on simple matching coefficients between varieties. The results showed that 44 SSR primer pairs were found to produce 176 bands , and 107（60.8%）were polymorphic. On an average, each primer pair produced 2.43 polymorphic bands in a total of 4 bands. The polymorphic percentage and PIC ranged from 11.1%~100% and 0.245~0.886, respectively. The average of PIC was 0.677.The result of cluster analysis indicated the majority of 12 accessions could be divided into three groups（Ⅰ、Ⅱ、Ⅲ）. The result showed that microsatellites are very useful DNA marker to analyze DNA polymorphism in cultivated peanut..The high genetic similarity of Shandong peanut at DNA level indicated gene bases, which need broadening in cultivar breeding through utilization of elite exotic germplasm resources.

CLC Number:

S565.202.4

Huang Xinyang, Wang Chuantang, Yang Xindao, Xu Jianzhi. Analysis of DNA Polymorphism in Peanut Cultivars Using New Microsatellite Markers[J]. Chinese Agricultural Science Bulletin, 2006, 22(10): 44-44.

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