Abstract: The cystglands of matured muskrat in secreting or the cystglands induced secreting artificially were used. Based on the morphological observation, epithelial cells of the scent glands were isolated and cultured in vitro initially. The results showed that a great quantity of dispersed cells in single state was available for primary culture by digestion with 1mg/ml Collagenase IV for 5 h and 0.25mg/ml Trypsin for 15min at 37℃; DME/F12 containing 10% fetal bovine serum, penicillin(100U/ml)and streptomycin(100U/ml), as well as EGF at the concentration of 10ng/ml, could obviously improve the survival and proliferation of these cells; the epithelial cells could be serial subcultivated, freezed and thawed routinely as well; after the 5th passage, the epithelial cells were filled with many lipid dropleds, secreting and proliferating perfectly. These results suggest that it is feasible to produce artificial muskrat scent using cell engineering by isolating, culturing cystglanitd epithelial cell and furthmore establishing a musdrat cystgland epithelial cell line.