Abstract: In view of the density and exudates content of glandular trichome depending on different developmental stages in flue-cured tobacco, the density and exudates of glandular trichome on leaves of flue-cured tobacco K326 with different stalk positions were investigated with wide visual microscope and GC/MS. The results showed that the density of glandular trichome of mature leaves was lowest in lower leaves, the second in cutter leaves, and the highest was found in upper leaves. α-Duvatriene-4-ol, β-Duvatriene-4-ol, α-Duvatriene-4,6-diol and β-Duvatriene-4,6-diol were higher in cutter leaves than that in lower leaves by 282%, 240%, 48% and 80% respectively, while those in upper leaves were higher than that in lower leaves by 991%, 687%, 74% and 129%, respectively. This indicated both the density of glandular trichome and the major components of trichome exudates of leaves were increased along with the rising of leaf stalk positions.