Abstract: Based on soil microelement content conditions of pasture planting section in Henan province and the requirement characteristic of pasture nutrition and animal nutrition, this paper conducted the studies about the effect of the Combined Application of sevsral trace element fertilizers (Zn, Fe, Cu, Mn, B, Mo and Co) on microelement nutrition of alfalfa (Medicago sativa L), in order to provide high quality pasture of different use and containing abundant trace element. The results showed that the CD compounding could remarkably increase the Zn, Fe, B’s contents of the second cut Medicago and the Zn, Fe, Cu, B ’s contents of the third cut Medicag and enhance the element’s accumulating quantity of the every cuts except the Co element of the second cut. The C1D1 compounding could remarkably increase the Mo, Co, B’s contents of the first cut Medicago and the Zn, Mo, Co, B, Fe’s contents of the second cut Medicag and the Cu, Zn, B, Fe, Mn’s contents of the third cut Medicag and enhance the element’s accumulating quantity of the every cuts except the Fe element of the second cut. The C1D2 compounding could remarkably increase the Zn, Mo, Co’s contents of the first cut Medicago and the Zn, Mo, Co, B, Fe, Mn’s contents of the second cut Medicag and the Zn, Co, B, Fe, Mn’s contents of the third cut Medicag and enhance the element’s accumulating quantity of the every cuts except the Fe element of the second cut. The C2D1 compounding could remarkably increase the Zn, Mo and Co’s contents of the first cut Medicago and the Cu, Zn, Mo, Co, B, Fe, Mn ’s contents of the second cut Medicag and the Fe, B, Mo ’s contents of the third cut Medicag and enhance the element’s accumulating quantity of the every cuts except the Fe element of the second cut. The C2D2 compounding could remarkably increase the Zn, Cu, Mo, Co’s contents of the first cut Medicago and the Zn, Fe, Cu, Mn, B, Mo and Co ’s contents of the second cut Medicag and the Zn, Fe, Mn, B, Mo and Co’s contents of the third cut Medicag and enhance the element’s accumulating quantity of the every cuts.