Abstract: The effect of different peat levels on soil physical and chemical traits and tobacco leaf yield and quality with pot experiment was conducted in 2005. The results showed that with the peat levels increasing from 250 to 2000g/plant, soil bulk density decreased, the decreasing range was 0.78~14.84%;however soil bulk porosity increased and increasing range was1.28~14.01%;soil organic matter and soil nitrogen also increased and increasing range were 21.15~99.36% and 3.80~35.65% respectively. Also with increasing the peat levels, the tobacco grew very fast during the early stage and tobacco leaf yield improved greatly when the peat levels were at 1000~2000 g/plant, but there was little effect when the peat level was less. The results also showed that with the peat levels increasing the leaf quality was improved. There was little effect on soil urease and catalase activity for different peat levels. In general, with peat levels increasing, soil bulk density decrease and soil bulk porosity increased and so as soil organic matter and soil nitrogen, the peat also improved tobacco growth and leaf yield and quality.