Abstract: Idesia polycarpa Maxim. is an plant with highly appreciative and useful value.This experiment concerning Idesia polycarpa Maxim.segment culture in vitro was committed on the explant sterilization, shoot vitrification control, shoot multiplication and root induction. The results showed that：(1)as for the one-year-old mother tree acclimated at Nanjing, Jiangsu Province, formerly introduced from Japan, the latter of April was optimal time in explant collection. After 0.1% HgCl2 sterilization for 15s，the explants suitful for the forthcoming culture accounted up to 76.7%；(2)addition of 3.5% sugar and air-permeation improvement to culture containers could effectively controll shoot vitrification；(3)the modified MS media supplemented with TDZ, BA and NAA could ameliorate the culture multiplication and growth,among which the combination of 0.05mg/L TDZ + 1.5 mg/L BA + 0.05 mg/NAAL was optimal, with the multiplication time up to 5.3 and growth height up to 2.7cm；(4) the rooting medium, i.e. 1/2MS+ 0.5 mg/L IBA + 0.3 mg/L NAA + 100 mg/L LH + 2% S was optimised ，with rooting percentage up to 87%；(5) the root-developed plantlets transplanted in the substrate mixed with vermiculite, perlite and peat (volumn ratio of 6:3:1) at 20~30℃, relative humidity of 85%~90% for15~20d, favorably shaded, could get over 85% survival after 30d.