Abstract: To provide the theoretical basis of the successful introduction of Rhododendron fortunei for the disposition in garden and the management in different growth stage, the eco-physiology indexes of Rhododendron fortunei in the different growth stage and the different place was measured by the LI-6400 portable photosynthesis. The result indicated that the curve of diurnal variation of net photosynthetic rate (Pn) was dual-peaked, but the average Pn of the seedlings were smaller than the mature trees and the seedlings in the introduction place were smaller than in the original habitat. The transpiration rate (Tr) of mature trees had reached the peak at 12:00, but the seedlings were at 14:00. The analysis of correlation showed that RH had negative correlation with Pn and Tr. Temperature, PAR and CO2 density had positive correlation with Pn and Tr.