Abstract: Using soil culture and micro-field experiment, we study the effects of controlled release nitrogen fertilizer (urea formaldehyde UF) on growth, physical index, output and fertilizer utilization ratio of the lettuce by comparing the controlled release nitrogen fertilizer with commercial quick-acting N fertilizers at different available N level. The result shows that UF was superior to commercial N fertilizers on growth index and output of lettuce, which had the order of UF > CO (NH2)2 > NH4NO3 > NH4HCO3. The results also indicated that UF had the highest N utilization ratio, which was up to 40.10%, while the other commercial N fertilizers increased their N utilization ratio from 6.93% to 31.04%. The amount of UF is closely related to the output of the lettuce and the medium level of N had the best fertilizer efficiency. If fertilizers supply excessively, the efficiency of the fertilizer decline significantly, and environmental concern has also risen.