Abstract: Twenty pumpkin inbred lines were chosen to analyse the content of K, Ca, P, Mg, Cu, Fe, Zn, sucrose, glucose, fructose, sugar content, β-carotene, solide, protein, cellulose, starch, acid, Vc. Relationship and variation of different pumpkin inbred lines’ quality traits were investigated. The results indicated that the Correlation coefficients of Mg and K, Ca, P, Cu, Ca and Zn, sucrose and sugar content, solide, glucose and fructose, β-carotene and Vc, protein were at the significant or very significant level. β-carotene has a maximum of variation in the quality traits, but Cellulose has a minimum of variation in the quality. The basis throes was made for early selection of pumpkin breeding, utilization of pumpkin germplasm resources.