Abstract: The stem-segment with axillary buds of Dendrobium bellatulum Rolfe as the explants, were cultured in tubes. The results of experiments suggested that appropriate media for respective culture stages were: (1) The germinaton of axillary buds: Ms + 6-BA5mg/L +NAA0.4mg/L (2) The differentiation and subculture Ms + 6-BA2 mg/L +NAA0.2 mg/L (3)Rootage: 1/2 MS+NAA0.2mg/L+PP333 was optional basal medium for proliferation , the rooting speed, number and percentage of adventitionous buds placed the medium levelly were significantly higher than thouse placed uprightly.