Abstract: The state of tube seedlings of cherry in vitro was improved remarkably by increasing concentration of sugar in medium properly. Proliferation rate was 1~2 times to 25~30 times that concentration of sugar from 2% to 6% in F14 medium and color of leaf from dim yellow-green into luster deep green,keep life from 15 days to 70 days. Adventitious shoots were induced from leaves after processed 4 stages culture.The regeneration rate is 91%. It was made a comprehensive survey of that ① The distinguishing feature tube seedlings which leaves were little and shoots were exuberant were gained by inducing common sweet cherry subculturing tube seedlings by planted them to F14 medium (addition 6-BA0.5mg/L+IBA0.05mg/L+GA30.2mg/L+sucrose6%+agar7.0g/L，pH5.8) for 30 days. ②The expanded and furled leaves were collected from the shoots. Each immature leaf was cut several times just across the midrib then droped it in 0.1%VC sterile water immediately and kept 1-30minutes so as to absorb water and prevent to be oxidized,place them on MS,F14 or WPM medium（addition 6-BA2mg/L+2，4-D2mg/L+sucrose 4%+agar7.0g/L，pH5.8）for 3~4days.then tissues of edge of leaves were dedifferentiated. ③placed leaves on WPM medium modified only with 1/2 marco（addition 6-BA2mg/L+IAA2mg/L+sucrose 4%+agar7.0g/L，pH5.8）and enviroment control(light intensity at 2000lux and illuminated for 15h/d.temperature was controled at 20℃ in light period and 15℃ in dark)for 20-30 days. Colorless and translucent callus were induced in beginning then it’s color turn into red with progressively rich for endogenous anthocyanins were induced.④placed leaves on F14 medium（addition 6-BA0.5mg/L+IBA0.05mg/L+GA32mg/L+ sucrose 6%+ agar 7.0g/L，pH5.8）for 20 days.then adventitious shoots were regenerated from red callus and callus’s red color was fading.