Abstract: Factors including dry method, comminution and extracting effect on the extraction amount of condensed tannin in the leaf of Lycium ruthenicum Murr were studied. To get the detail relationship among these parameters, different level of those factors was set when measuring the condensed tannin, and the results were analyzed by statistical methods. The results show that both the main effect of a single factor and the mutual action of the factors existed, especially the action between the dry method and the extract, and the action between the commimution and the extract. Freezing dry was better than air dry, medium size particle was better than the large and small particles and the acidic methyl alcohol solution was better than that without acid. Of all the treatments, the combination of freezing dry, 80~100 mesh sieve and 1% HCl in 70% methyl alcohol could yield the largest amount of condensed tannin from the leaf of Lycium ruthenicum Murr.