Amplification and Sequencing of Exotoxin A Structural gene PE40 of Pseudomonas Aeruginosa ATCC27853

Abstract

Abstract: Objective: To analyse nucleotide sequence of exotoxin A structural gene PE40 of Pseudomonas aeruginosa ATCC27853. Method: The gene PE40 was amplified from genome DNA of Pseudomonas aeruginosa ATCC2785 by PCR technique. The PCR product was purified, sequenced and compared with a standard strain PA103 from GenBank by DNASTAR software. Result: An objective gene fragment with the length 1231bp was obtained. The nucleotide sequence analysis showed that ATCC27853 shared 98% alignment with PA103. The mutations in amino acid sequence are: Asn-364 to Ser; Ser-506 to Trp and Ser-515 to Gly. Conclusion: The mutations have a little influence on the enzymatic activity and cytotoxicity, so the gene PE40 of Pseudomonas aeruginosa ATCC27853 will be usefull in the research of molecular targeted protein.

CLC Number:

Q781

Gu Xiaolong, Qin Jianhua, Zhang Ning, Guo Li. Amplification and Sequencing of Exotoxin A Structural gene PE40 of Pseudomonas Aeruginosa ATCC27853[J]. Chinese Agricultural Science Bulletin, 2006, 22(8): 30-30.

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