Abstract: To study double-cropping seeds production techniques of flue-cured tobacco MS hybrid, experiments were carried out on the ratio of male parent to female parent and fertilization level in the first crop season. Comparison was carried out between MS hybrid seeds production in the fist crop season and in the second crop season in output and quality of hybrid seed，as well as their seed production cost and laborer employed. The results showed that the best ratio of male parent to female parent was 1:3, and 210 kg/hm2 N fertilization was better. It indicated that hybrid seeds production in two crop seasons, especially the second crop season based on the utilization of ratoons of flue-cured tobacco which regenerated after hybrid seed was harvested in the first crop season, was beneficial to higher seed yield, lower cost, and less laborers employed as well.