Abstract: The leaves of fast growing poplar were used as explants and a in vitro culture system was established. The results showed that the leaf vein and petiole produced adventitious buds very easily and most buds occurred directly in the leaves; the BA concentration in media is the key to the bud formation; when the concentration was 0.5~2.0mg/L BA, the adventitious buds occurred, and combining 1.0mg/L BA with0.05mg/l NAA was beneficial to the adventitious bud formation; adding 0.1~0.2mg/L GA3 to the media could increase the Frequency of producing the adventitious buds. Use the propagation medium MS+0.5mg/L BA+0.05mg/L NAA +0.05mg/L GA3 could not only realize rapid propagation but also make the plantlets robust and the verifications rate lower. Adopting transition culture in rooting had a better result, and adding ABT3-rooting-powder to the media made the quality of test-tuber plantlets much better. The in vitro regeneration system of leaves of the poplar was established based on the experimental results. Using this system could reach a high regeneration frequency but different varieties would differ a little bit.