Abstract: Total RNA was extracted from the top leaves of red forma of C.blumei by using Plant Total RNA Extraction kit, and cDNA library was constructed with SMART cDNA Library Construction Kit. The titer of the primary library was 1.2×106 pfu/ml, in which 98% clones were recombinant and the inserted cDNA fragments ranged from 0.5 kb to 2 kb, 60% of them more than 1kb. The amplified library has a titer of 6.7×109 pfu/ml. The positive signals of CHS, DFR and ANS gene were detected by PCR in the amplified library. The data indicate that the cDNA Library has high quality, and provides a useful tool for cloning leaf color genes and further study on the molecular mechanisms of the pigments biosynthesis of the flavonoids in C.blumei.