Chinese Agricultural Science Bulletin ›› 2008, Vol. 24 ›› Issue (10): 22-25.
Special Issue: 生物技术; 畜牧兽医
• 畜牧兽医科学 • Previous Articles Next Articles
ZHAO Xu-Yong
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Abstract: The SIgM λ light chain gene about 630bp was obtained from the total RNA of the chicken bursal B lymphocyte by RT-PCR , then the SIgM λ light chain gene was subcloned into the downstream of T7 promoter of the vector pET-28a to construct expression vector of pET SIgM λ. The recombinant plasmid was transformed into in E. coli BL21 (DE3) and induced with IPTG. SDS-PAGE and Western blotting analyses showed that the chicken SIgM λ light chain protein of 25.5 kDa were efficiently expressed and mainly existed as inclusion bodies ,with specific reactivity to anti-His antibodies. Moreover,it may be helpful to study its Immunologic and other biological functions.
ZHAO Xu-Yong. Expression and Identification of the expression of chicken surface IgM λ light chain in E.Coli[J]. Chinese Agricultural Science Bulletin, 2008, 24(10): 22-25.
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https://www.casb.org.cn/EN/Y2008/V24/I10/22