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Chinese Agricultural Science Bulletin ›› 2008, Vol. 24 ›› Issue (11): 78-80.doi: 10.11924/j.issn.1000-6850.2008-0662

Special Issue: 生物技术； 小麦

• 农业生物技术科学 • Previous Articles     Next Articles

Recombinant expression vector with wheat MBD3 gene and its prokaryotic expression

Meng Fanrong, Si Zhifei, Liu Haoying, Zhang Wen   

  • Received:2008-08-12 Revised:2008-09-08 Online:2008-11-05 Published:2008-11-05

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Abstract: In this study, a prokaryotic expression vector pGEX-4T-MBD3 for the wheat MBD (Methyl-binding domain protein) gene MBD3 was constructed. Transform it to E. coli. BL21 (DE3), the fusion protein GST-MBD3 with molecular weight of 49.6 kDа was effectively expressed under 1mM IPTG concentrations at 37°C, which provides a foundation for further purification and functional study of the MBD3 protein.