Abstract: Mulberry (Morus spp) is the optimized species in agricultural structure adjustment of China and has a high economic value. The seedling of mulberry can be propagated rapidly by tissue culture. The author focused on the effects of various sterilization conditions, different concentration of mediums and different browning inhibition on tissue rapid propagation using Mulberry’s side bud tissue as explants. As a result, the explants have grown into healthy and strong callus and been inducted into roots. The main results were as follows: The Mulberry’s side bud tissue was disinfected for 30 seconds using 75% alcohol firstly, then for 8 minutes by HgCl2 solution with a few of Tween-80. In the culture medium of MS+6-BA 2mg/L + IAA 0.5mg/L, the induction rate of callus was about 80%. In the rooting culture medium of MS + IAA 0.1mg/L + 6–BA 0.5mg/L, the rooting rate was about 70%. If taking adventitious bud induction directly, each stem can produce three adventitious bud averagely in the culture medium of MS +IAA 0.2mg/L + 6–BA 3.0mg/L. Ascorbic acid can inhibit callus browning to a certain extent. But there are still many problems in the process of Mulberry’s tissue rapid propagation and further study is necessary.