Abstract: 【OBJECTIVE】Studying the correlation of pathogenicity and β-D-glucosidase activityof strains in order to find the fast testing method of the pathogenicity of Fusarium oxysporum from cucurbitaceous crops.【METHOD】we tested the pathogenicity and β-D-glucosidase activity for nineteen strains isolated from cucumber, melon and watermelon, and form a correlation construct of pathogenicity(y) and β-D-glucosidase activity(x).【RESULTS】The results showed that significant differences of the pathogenicity and β-D-glucosidase activity were found among different strains from the same or the different host-plants. The tested strains were grouped into pathogenicity and nonpathogenicity, and the β-D-glucosidase activity of strains from cucumber, melon and watermelon were in the range of 21.57-54.89，16.28-41.83 and 14.98-32.43 U•ml-1, respectively. The average activity of the strains from cucumber, melon and watermelon were 34.08, 27.86, 20.95 U•ml-1.【CONCLUSION】There was a positive correlation between the β-D-glucosidase activity and pathogenicity of F. oxysporum isolated from cucurbitaceous crops while the activity was less than 30 U•ml-1; contrarily , disease incidence of strains were more than 90% while more than 30 U•ml-1.