Abstract: The effects of root excision on the root morphology, root hydraulic conductivity（Lpr）and leaf photosynthesis, leaf transpiration of maize seedlings were studied under condition of solution culture. The results showed that after root excision, the roots hydraulic conductivity of maize seedlings was improved, and photosynthesis rate, transpiration rate and stomatal conductance of leaf were decreased. Of the physiological indexes, the transpiration rate was influenced by root excision most. Length, surface area and tips of root system were also decreased with root excision, while average root length was increased under root excision. There was no significant difference between root excision and check for average diameter of roots.