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Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (17): 20-24.

Special Issue: 玉米 水稻

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Study on Analysis Method of Melatonin and Melatonin content in corn & rice seeds

Wang Jinying1, Jiang Chuan1, Li Shuke1, Zheng Jingui2   

  • Received:2009-03-09 Revised:2009-04-02 Online:2009-09-05 Published:2009-09-05

Abstract:

Abstract:Melatonin(MT for short) is a indole-trytamine(N-Acetyl-5-hydroxy tryptamine). It was verified that MT exists almost all biological organism. The seeds of corn and rice wrer utilized as the materials of the experiment, study on analysis method of melatonin and MT content in seeds of corn, rice. The results of this study were as follow: (1)It was practicable to detect MT content in plant seeds by the method of HPLC. The regression equation was Y=643436X-2446.1 and R2=0.9998,when the concentration of melatonin was 0.01~1.20mg/kg. The average recovery rate was 94.11%.(2)It was indicated that MT exists in the seeds of corn and rice,and the MT concentration of various species and varieties was significant different. Average MT content of corn seed was 96.5 PPb, it’s range was 0-2034 PPb ; Average MT content of rice seed was 16 PPb, it’s range was 0-264 PPb.