Abstract:

In this paper, we discussed the fusion-PCR by adding self-extension process. The self-extension procedure was cycled for 2 times. When the extension length of self-extension procedure was 2, 3, or 5 minutes, the fusion-gene band amplified by fusion-PCR was significantly brighter than that of traditional PCR. However, when the extension length of the self-extension was 1 minutes, the genes amplified were similar between these two PCR processes. This indicates that the extension length of self-extension procedure is an important factor influencing gene amplified in Fusion-PCR.