Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (24): 62-67.
Special Issue: 生物技术
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Abstract:
A total length DFR gene was cloned with RT-PCR and RACE technology from flower bud with blue petals of Hyacinthus orientalis L.. Hyacinthus DFR cDNA was 1252 bp in length.Within the f ull-length cDNA , a clear open reading frame (ORF) was 1098 nucleotides ,coding 366 amino acids. The result s of homologous analysis in GenBank demonstrated that the sequence had 66 %~77 % identity on the nucleotide sequence and 62 %~73% identity on the deduced amino acid sequence. The results of phylogenetic analysis showed that DFR from Hyacinthus orientalis and from Iris x hollandica clustered together firstly ,and then came those from other monocotyledon, dicotyledon came subsequently.
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https://www.casb.org.cn/EN/Y2009/V25/I24/62