Abstract: Taking stem as the primary explants, a tissue culture system was established by using medium added with hormone at different concentration for two provenances of Siberian elm obtained from Cangzhou, Hebei; Chifeng, Neimeng. The results showed that the best differentiation medium for stem from Cangzhou is MS +6-BA 0.10㎎/L+IBA0.005㎎/L and for stem from Chifeng is MS+6-BA 0.10㎎/L+IBA0.025㎎/L; the best rooting medium for stem from Cangzhou is MS +IBA0.01㎎/L and for stem from Chifeng is MS +IBA0.05㎎/L. MS medium supplemented with 0.005mg/L TDZ and 0.005㎎/L IAA was the optimal medium condition of leaf regeneration of these two Siberian elms.