Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (5): 18-21.
Special Issue: 生物技术; 畜牧兽医
• 畜牧兽医科学 • Previous Articles Next Articles
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Abstract: The present experiment was conducted to assess DNA extraction method of freezing-thawing and methods combined freezing-thawing and CATB. Results showed that the methods combination of freezing-thawing and CATB could smash cell wall of varieties of micro-organisms including bacteria and Fungus. DNA extraction was stable by this technique and DNA fragments were large enough to be applied in follow-up research. It could be concluded that freezing-thawing and CATB methods combination could extract the total rumen microbial DNA.
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https://www.casb.org.cn/EN/Y2009/V25/I5/18