Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (5): 18-21.

Special Issue: 生物技术 畜牧兽医

• 畜牧兽医科学 • Previous Articles     Next Articles

Extraction method optimizing of total DNA in rumen microorganism of Laoshan dariy

  

  • Received:2008-12-03 Revised:2009-02-04 Online:2009-03-05 Published:2009-03-05

Abstract: The present experiment was conducted to assess DNA extraction method of freezing-thawing and methods combined freezing-thawing and CATB. Results showed that the methods combination of freezing-thawing and CATB could smash cell wall of varieties of micro-organisms including bacteria and Fungus. DNA extraction was stable by this technique and DNA fragments were large enough to be applied in follow-up research. It could be concluded that freezing-thawing and CATB methods combination could extract the total rumen microbial DNA.