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Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (9): 26-31.

Special Issue: 水稻

• 农业生物技术科学 • Previous Articles     Next Articles

QTL Mapping for Cadmium Tolerance in Rice (Oryza sativa L.) Seedling

  

  • Received:2009-01-16 Revised:2009-02-12 Online:2009-05-05 Published:2009-05-05

Abstract: [Objective]Research was done to detect the QTLs of tolerance to heavy metal cadmium in rice accessions.[Method](1) A recombinant inbred lines (RILs) including the population of 123 F10, which were derived from a cross between Indica rice Lemont(Cd Tolerance,USA)and Japonica rice Dular(Cd sensitive rice, India)was employed to investigate the genes in rice related to heavy metal cadmium (Cd) stress. All rice lines including parents were grown in Hoaglland solution with 0.2mg/L additional cadmium, and all entries grown in Hoaglland solution without additional cadmium were serviced as control. (2)The chlorophyll content (CC), root length (RL), shoot heigh (SH), leaf length (LL) were determined and transformed to Index of Cd Tolerance (ICT) for QTL location involving 109 SSR markers by composite interval mapping (CIM). Nine additive QTLs were detected and mapped to chromosome 1, 2, 3, 11, respectively. Three QTLs, which were evaluated by ICT from the CC and located on chromosome 2, 3 and 11, explained 14%, 9% and 9% of the phenotypic variation, respectively. One QTL, which was detected by ICT from the RL and mapped to chromosomes 1, accounted for 9% of the phenotypic variation. Three QTLs, which were evaluated by ICT from the SH and mapped to chromosomes 1,1,11, contributed 10%, 27%, 10% to the total phenotypic variation. Two QTLs by the ICT from the LL were located on chromosome1 and 11, and accounted for 21% and 12% of the phenotypic variation. The result showed that seven out of nine QTLs associated with Cd tolerance were located on chromosome 1 and 11, of which 4 QTLs were on chromosome 1 and 3 QTLs were on chromosome 11. (Conclusion)It was found that that some QTLs associated with Cd tolerance were located in the same marker intervals on chromosome 1 and 11, implying that they maybe different alleles with the same functions or may be the same gene in different performent manners. At the same time, it suggested that the indexes was approciate and feasible.