Abstract:

In this paper，an experiments was carried with the bud of current year after certain treatment, followed the approach: stem→ PLB inducing→ PLB proliferating. Adopted single-factor, double-factor and orthogonal design methods, and studied the effects of the different treatments and sterilization time on the induction of PLB (protocorm-like body). The results were as following: The best sterilizing method for stem was disinfected with 70% alcohol for 3s, and then treated with 0.1% HgCl2 for 8 min. The formula of the optimal culture medium for PLB induction was the MS＋6-BA0.5 mg/L + NAA 0.5 mg/L + 2,4-D1.0 mg/L. The best medium for PLB proliferation was MS＋6-BA3.0mg/L+ NAA0.3mg/L.