Abstract:

Antimicrobial peptides are the most important substance for insect in protecting themselves from infection of microorganism. In the paper, the plant-specific promoter CaMV35S from pBI121 was constructed into pCAMBIA2300, the recombinant expression vector named pCAMBIA2300-CaMV35S, attacin full-length coding region (GenBank ccession number: GU244351) as amplified by PCR from Bombyx mori and then cloned into the pMD19-T simple vector. The positive clone cutted by pairs of restriction enzymes was successfully linked with recombinant expression vector pCAMBIA2300- CaMV35S. The recombinat plasmid of attacin was identified by PCR digestion. That lay the foundation for applications of the antibacterial peptides attacin gene in disease resistance of plant.