Abstract:

The genetic relationships among 22 Flammulina velutipes strains were analyzed by SRAP primers. Nine pairs of primers with good polymorphism were selected to amplify 22 representative F. velutipes strains from different sources. 189 repeatable DNA bands from tested F. velutipes were obtained. A dendrogram constructed by using the amplification data separated the test strains into five groups at a coefficient of 0.820. Group I comprised F. velutipes F21 gaoyou, F. velutipes F43, F. velutipes F1312 gaoyou, F. velutipes F45, F. velutipes F48, F. velutipes jinbai no. 10, F. velutipes 2001, F. velutipes baijinshangzhuang, F. velutipes jinbai no. 1, F. velutipes jinbai no. 8; Group II comprised F. velutipes rijin no. 1, F. velutipes F47. Group III comprised F. velutipes F8909, F. velutipes F14, F. velutipes Ftian, F. velutipes F hefei, F. velutipes FfenP, F. velutipes FA2, F. velutipes F49, F. velutipes F6. Group IV comprised F. velutipes 2098 and group V comprised F. velutipes Japan jinxin. Our data showed that SRAP could reveal the genetic diversity of F. velutipes strains in beijing region.