Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (11): 32-35.

Special Issue: 生物技术 水稻

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The Rapid Extraction of Rice Genomic DNA from Natural Dried Tissue

Tian Yonghang, Luo Hongfa   

  • Received:2010-01-13 Revised:2010-02-19 Online:2010-06-05 Published:2010-06-05

Abstract:

After the alkali treatment for rice natural dried tissues, taking the alkaline leaching solution directly as a template for PCR amplification, the amplification results are stable, accurate, and are insignificantly different in comparison with the methods CTAB and SDS. The DNA extracted from natural dried rice flag leaf, leaf sheath ,culm ,glume husk and roots ,have good quality, and can meet with the SSR analysis.