Abstract:

Abstract: Immobilized microbiological fertilizer could extend the shelf-life and maintain the bacterium purity, however, the price of informed immobilization material was too high for large-scale production and unsuitable for all microbiological fertilizer strains. In this paper,immobilization of Bacillus megaterium,Bacillus mucilaginosus and Azotobocter chroococcum by entrapping agents was studied,in order to find the optimal material and the technique for the immobilization of the three bacterium, two formula invoved four materials was designed to test the survival and release rate of the three bacteriums ,therefore the possibility of immobilization of the three bacteriums used for microbiological fertilizer could be further discussed. Experiment result showed that the characteristics of the immobilization cells of the two formula was quite different. For the immobilization capacity of the three bacteriums, the better material was 3% sodium aginate +1% flyash +0.5% soluble starch, For the purity of the three bacteriums, 3% sodium aginate +1% flyash +0.5% soluble starch was better. miscllaneous bacterium tend to grow more in the formula of 3% sodium aginate +1.5% flyash +0.3% soluble starch +0.3% gelatin than the formula of 3% sodium aginate +1% flyash +0.5% soluble starch.and for the survial of Bacillus sp.,both formula was better than the formula of sodium aginate.