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Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (12): 221-226.

Special Issue: 生物技术； 园艺

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Identification and the internal transcribed spacer regions analysis of pathogen causing rubber anthracnose

Li Jifeng1,2, LiuXianbao2, Cai Jimiao2, Lin Chunhua2, Huang Guixiu2   

  • Received:2010-01-04 Revised:2010-01-12 Online:2010-06-20 Published:2010-06-20

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Abstract:

Abstract: The internal transcribed spacer regions (5.8S/ITS) of the ribosomal RNA gene from 22 isolates of colletotrichum leaf disease of rubber were amplified through the polymerase chain reaction with the universal primers and sequenced. And their biological characters also tested. The result revealed that only 7 isolates belong to Colletotrichum gloeosporioides. The remaining 15 isolates represented Colletotrichum acutatum. Intraspecific genetic variation of Colletotrichum acutatum isolated from rubber was relatively low, which was found through rDNA-ITS sequence analyses. However, intraspecific genetic variation of Colletotrichum gloeosporioides was relatively abundant.