Abstract:

In order to establish a two-dimensional gel electrophoresis (2-DE) protocol for proteomic study of Lactuca sative L., with leaves of Lactuca sative L. (S24) as material, we used TCA / acetone precipitation method extracted leaf protein, compared the two kinds of standard curve for protein concentration determination of the impact and also discussed on the lysis method , process of IEF and some key technologies of 2D electrophoresis. The results showed that: Compared with the traditional standard curve, using the improved standard curve more accurate determination of protein concentration. A two-dimensional electrophoresis atlas with good repeatability and high resolution was obtained. A system of two-dimensional electrophoresis of leaves of Lactuca sative L.was successfully established.