Abstract: The bacteriawere isolated from Carp,the physiobiochemical characteristics and the antimicrobial susceptibility assay were carried out. CC-1 and CC-2 strains can produce phenylalanine dehydrogenate in the biological and chemical identification of bacteria and can produce gas production of glucose. CC-1 strain can produce the lysine decarboxylase and the ornithine amino acid decarboxylase, but CC-2 strain cannot. CC-1 and CC-2 strains were highly sensitive to cephalosporin, aminoglycoside, macrolide and sulfonamide antibiotic, but its had different levels of drug resistance to ampicillin,rifampicin,naphthaleneacetic acid,cephalothin and cephalosporin. This study may be provide a scientific reference in the treatment of fishes of disease and for reasonable development and utilization of Bacteria in Carp intestinal resources.